Preparation of Silica-Encapsulated Hollow Gold Nanosphere Tags Using Layer-by-Layer Method for Multiplex Surface-Enhanced Raman Scattering Detection

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Year 2011 
Journal Info. Langmuir, 27, 10228-10233 (2011). 
Author Jianshe Huang, Ki Hyung Kim, Namhyun Choi, Hyangah Chon, Sangyeop Lee and Jaebum Choo* 
etc.  

Abstract

The use of silica shells offers many advantages in surface-enhanced Raman scattering (SERS)-based biological sensing applications due to their optical transparency, remarkable stability in environmental media, and improved biocompatibility. Here, we report a novel layer-by-layer method for the preparation of silica-hollow gold nanosphere (HGN) SERS tags. Poly(acrylic acid) was used to stabilize Ramanreporter tagged HGNs prior to the adsorption of a coupling agent, after which a silica shell was deposited onto the particle surface using Stober’s method. Importantly, competitive adsorption of the Raman reporter molecules and coupling agents, which results in unbalanced loading of reporter molecules on individual nanoparticles, was avoided using this method. As a result, the loading density of reporter molecules could be maximized. In addition, HGNs exhibited strong enhancement effects from the individual particles because of their ability to localize the surface electromagnetic fields through pinholes in the hollow particle structures. The proposed layer-by-layer silica-encapsulated HGN tags showed strong SERS signals as well as excellent multiplexing capabilities.

 

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Year Title Journal Info. etc.
2011  Time-lapse, single cell based confocal imaging analysis of caspase activation and phosphatidylserine flipping during cellular apoptosis file Biotechnic & Histochemistry, 86, 181-187 (2011).   
2011  Preparation of Silica-Encapsulated Hollow Gold Nanosphere Tags Using Layer-by-Layer Method for Multiplex Surface-Enhanced Raman Scattering Detection file Langmuir, 27, 10228-10233 (2011).   
2011  Real-time detection of cellular apoptosis using a rat C6 glioma cell-based assay system file Molecular & Cellular Toxicology, 7, 177-184 (2011).   
2011  Ultraviolet Absorption Spectra, Structure, Vibrations, and Theoretical Calculations of 2-Fluoro- and 3-Fluoropyridine in their Electronic Excited States file Chemical Physics Letters. 514, 214-219 (2011).   
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2011  Recent advances in microfluidic technologies for biochemistry and molecular biology file BMB reports, 44, 705-712 (2011).  review paper 
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2011  Polymorph-tuned Synthesis of a- and b-Bi2O3 Nanowires and Determination of Their Growth Direction from Polarized Raman Single Nanowire Microscopy file Chemistry - A European Journal, 17, 1304-1309 (2011).   
2011  Au Nanowire-on-Film SERRS Sensor for Ultrasensitive Hg2+ Detection file Chemistry - A European Journal, 17, 2211-2214 (2011).   
2011  Pep-1 Peptide-Modified Liposomal Carriers for Intracellular Delivery of Gold Nanoparticles file Chemical & Pharmaceutical Bulletin, 59, 109-112 (2011).   
2011  Pt nanoparticle-supported multiwall carbon nanotube electrodes for amperometric hydrogen detection file Sensors and Actuators B, 155, 191-198 (2011).   
2011  Enzyme kinetic measurements using a droplet-based microfluidic system with a concentration gradient file Analytical Chemistry, 83, 1603-1608 (2011).   
2010  Fabrication of protein-anchoring surface by modification of SiO2 with liposomal bilayer file Colloids and Surfaces B: Biointerfaces, 75, 209-213 (2010).   
2010  Optofluidic platforms based on surface-enhanced Raman scattering file Analyst, 135, 837–844 (2010).  Cover page 
2010  Preparation of Targeting Proteoliposome by Postinsertion of a Linker Molecule Conjugated with Recombinant Human Epidermal Growth Factor file Bioconjugate Chemistry, 21, 345-351 (2010).   
2010  Nanomaterial-assisted aptamers for optical sensing file Biosensors and Bioelectronics, 25, 1859–1868 (2010).   
2010  High sensitive detection of C-reactive protein by total internal reflection fluorescence microscopy on rapidly making nanoarray protein chip file Talanta, 81, 1402–1408 (2010)   
2010  Effect of cholesterol content on affinity and stability of factor VIII and annexin V binding to a liposomal bilayer membrane file Chemistry and Physics of Lipids, 163, 335-340 (2010).   
2010  Electrochemical Patterning of Transparent Single-Walled Carbon Nanotube Films on Plastic Substrates file Langmuir, 26, 9136-9141 (2010).   
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