On-chip Immunoassay Using Surface-Enhanced Raman Scattering of Hollow Gold Nanospheres

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Year 2010 
Journal Info. Analytical Chemistry, 82, 5290–5295 (2010). 
Author Hyangah Chon, Chaesung Lim, Seung-Mo Ha, Yoomin Ahn, Eun Kyu Lee, Soo-Ik Chang, Gi Hun Seong and Jaebum Choo* 
etc.  

Abstract

A surface-enhanced Raman scattering (SERS)-based gradient optofluidic sensor has been developed for a fast and sensitive immunoassay. In this work, a novel microfluidic sensor with functional internal structures has been de- signed and fabricated. This sensor is composed of three compartments consisting of the gradient channel that serially dilutes the target marker, the injection and mixing area of antibody-conjugated hollow gold nanospheres and magnetic beads, and the trapping area of sandwich im- munocomplexes using multiple solenoids. Quantitative analysis of a specific target marker is performed by analyzing its characteristic SERS signals. This SERS-based gradient optofluidic sensor can replace the set of microw- ells or microtubes used in manual serial dilutions that have been traditionally used in enzyme-linked immun- osorbent assay (ELISA)-type assays. The limit of detection for rabbit immunoglobin (IgG) is estimated to be 1-10 ng/mL. This novel SERS-based optofluidic immunoassay system is expected to be a powerful clinical tool for the fast and sensitive medical diagnosis of a disease.

 

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