Quantitative Serodiagnosis of Scrub Typhus Using SERS-Based Lateral Flow Assay Platforms

Manager 2019.07.30 12:57 Views : 8370

Year 2019 
Journal Info. Analytical Chemistry, 91, 12275-12282 (2019) 
Author See Hi Lee, Joonki Hwang, Kihyun Kim, Jinhyeok Jeon, Sangyeop Lee, Juhui Ko, Jicheol Lee, Minhee Kang, Doo Ryeon Chung andJaebum Choo* 
etc. Cover page 

A surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) technique has been developed for the rapid and accurate diagnosis of scrub typhus. Lateral flow kits for the detection of O. tsutsugamushi IgG (scrub typhus biomarker) were fabricated, and the calibration curve for various standard clinical sera concentrations were obtained by Raman measurements. The clinical sera titer values were determined by fitting the Raman data to the calibration curve. To assess the clinical feasibility of the proposed method, SERS-LFA assays were performed on 40 clinical samples. The results showed good agreement with those of the standard indirect immunofluorescence assay (IFA) method. SERS-LFA has many advantages over IFA including the less sample volume, simpler assay steps, shorter assay time, more systematic quantitative analysis, and longer assay lifetime. As SERS strips can be easily integrated with a miniaturized Raman spectrophotometer, field serodiagnosis is also more feasible.

 

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Year Title Journal Info. etc.
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2019  SERS-based immunoassay using gold-patterned array chips for rapid and sensitive detection of dual cardiac biomarkers file Analyst, 144, 6533-6540 (2019)  Cover page 
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2019  Epitaxially aligned submillimeter-scale silver nanoplates grown by simple vapor transport file Nanoscale, 11, 17436-17443 (2019)   
2019  Quantitative Serodiagnosis of Scrub Typhus Using SERS-Based Lateral Flow Assay Platforms file Analytical Chemistry, 91, 12275-12282 (2019)  Cover page 
2019  Gold nanorods functionalized by a glutathione response near-infrared fluorescent probe as a promising nanoplatform for fluorescence imaging guided precision therapy file Nanoscale, 11, 12220-12229 (2019)   
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2019  Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxia stress file Journal of Materials Chemistry B, 7, 2557-2564 (2019)   
2019  Modification of Phage Display Technique for Improved Screening of High-Affinity Binding Peptides Journal of Biotechnology file Journal of Biotechnology, 289, 88-92 (2019)   
2019  High-throughput Microfluidic Imaging Flow Cytometry file Current Opinion in Biotechnology, 55, 36-43 (2019)   
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