Year 2021 
Journal Info. Sensors & Actuators B, 344, 130271 (2021) 
Author V. T. T. X. Ho, H. Park, S. An, G. Kim, N. H. Ly, S. Y. Lee, J. Choo*, H. S. Jung*, and S. -W. Joo* 


Intracellular Cu(II) in the human body is essential to many physiological functions, and its disruption is connected to several diseases. Synthetic coumarin–lipoic acid (Cou-LA) conjugate-functionalized silver-nanoparticles decorated on nanopipettes (CSNs) were fabricated herein for the determination of intracellular Cu(II) via in situ dual Raman/fluorescence spectroscopy. The CSNs selectively sensed Cu(II) over other metal ions to induce enhanced Raman intensities and fluorescence quenching. The determination of Cu(II) in single HeLa cells was achieved in accordance with changes in the ratio of Raman intensities at 500 and 597 cm-1 and fluorescence at 469 nm, which was ascribed to the capturing of Cu(II) by the CSNs. The Raman signals exhibited a good linear relationship with Cu(II) concentration from 10 to 75 μM with R2 = 0.956. The calibration curve indicated a local Cu(II) concentration of ~42.6 ± 8.6 μM in a single HeLa cell after pretreatment with 100 μM Cu(II) for 1 h. Cou-LA exhibited negligible cytotoxicity in both normoxic and hypoxic HeLa cells. However, a significant reduction in cell viability occurred with the Cu(II)–Cou-LA complex. This cytotoxicity was attributed to the generation of reactive oxygen species via a Cu-catalyzed Fenton-like process in tumor microenvironments and was found to be applicable to chemodynamic therapy (CDT). The system fabricated in this study represents a novel strategy for intracellular dual-mode Cu(II) detection and CDT applications in cancer research.

Year Title Journal Info. etc.
2021  Reproducible and sensitive plasmonic sensing platforms based on Au-nanoparticle-internalized nanodimpled substrate file Advanced Functional Materials, 31, 2105703 (2021)   
2021  SERS-based serodiagnosis of acute febrile diseases using plasmonic nanopopcorn microarray platforms file Biosensors & Bioelectronics, 192, 113525 (2021)   
2021  Coumarin–lipoic acid conjugates on silver nanoparticle-supported nanopipettes for in situ dual-mode monitoring of intracellular Cu(II) and potential chemodynamic therapy applications file Sensors & Actuators B, 344, 130271 (2021)   
2021  SERS-based test strips: Principles, designs and applications file Biosensors & Bioelectronics, 189, 113360 (2021)   
2021  Sensitive detection of SARS-CoV-2 using SERS-based aptasensor file ACS Sensors, 6, 2378-2385 (2021)   
2021  SERS-based microfluidic devices for in vitro diagnostics In "Principles and Clinical Diagnostic Applications of
Surface-Enhanced Raman Spectroscopy" Book Chapter 7, Elsevier, in press. 
Book Chapter 
2021  SERS-based Dual-flow Lateral Flow Assay Sensor for the Ultrasensitive Detection of Thyroid-Stimulating Hormone file Analytical Chemistry, 93, 6673-6681 (2021)   
2021  Molecular Imprinting: Green Perspectives and Strategies file Advanced Materials, 33, 2100543 (2021)   
2021  Recent advances in sensitive surface-enhanced Raman scattering-based lateral flow assay platforms for point-of-care diagnostics of infectious diseases file Sensors & Actuators B, 329, 129214 (2021)  Invited Review 
2021  Determination of total iron-binding capacity of transferrin using metal organic framework-based surface-enhanced Raman scattering spectroscopy file J. Raman Spectrosc., 52, 506-515 (2021)  Invited Paper 
2021  A Wearable Surface-Enhanced Raman Scattering Sensor for Label-Free Molecular Detection file ACS Applied Materials & Interfaces, 13, 3024-3032 (2021)   
2020  Recent advances in surface-enhanced Raman scattering-based microdevices for point-of-care diagnosis of viruses and bacteria file Nanoscale, 12, 21560 (2020)  Invited Review 
2020  SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A file Biosensors & Bioelectronics, 167, 112496 (2020)   
2020  SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing file Talanta, 220, 121397 (2020)   
2020  Improvement of reproducibility and thermal stability of surface-enhanced Raman scattering-based lateral flow assay strips using silica-encapsulated gold nanoparticles file Sensors & Actuators: B. Chemical, 321, 128521 (2020)   
2020  SERS biosensors for ultrasensitive detection of multiple biomarkers expressed in cancer cells file Biosensors & Bioelectronics, 164, 112326 (2020)   
2020  Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities file Biosensors & Bioelectronics, 165, 112282 (2020)   
2020  Multi-emitting fluorescence sensor of MnO2-OPD-QDs for the multiplexed and visual detection of ascorbic acid and alkaline phosphatase file Journal of Materials Chemistry C, 8, 5554-5561 (2020)   
2020  Analysis of deoxyribonuclease activity by conjugation-free fluorescence polarization in sub-nanoliter droplets file Analyst, 145, 3222-3228 (2020)  Cover page 
2020  Present and Future of Surface-Enhanced Raman Scattering file ACS Nano, 14, 28-117 (2020)