Year 2021 
Journal Info. Biosensors & Bioelectronics, 192, 113525 (2021) 
Author Anupam Das, Kihyun Kim, Sung-Gyu Park, Namhyun Choi*, and Jaebum Choo* 


We report a surface-enhanced Raman scattering (SERS)-based immunoassay platform for the rapid diagnosis of scrub typhus and murine typhus, which are the most common acute febrile diseases in South Korea. A microarray device, composed of multiple gold nanopopcorn substrates capable of detecting ultra-sensitive biomarkers, was used as a multiplex SERS-based assay platform. Sequentially diluted titers of Orientia tsutsugamushi and Rickettsia typhi specific human IgG/IgM antibodies, which are biomarkers of two typhus diseases, were analyzed by Raman spectroscopy, and the peak intensity was plotted against the different titer concentration range (0–2048 and 0–1024 for O. tsutsugamushi IgG/IgM and 0–8192 and 0–256 for R. typhi IgG/IgM) to generate calibration curves. The sensitivities and limits of detection (LODs) determined for four different IgG/IgM antibodies were significantly lower than those for the ELISA method. The LODs of titer concentrations for O. tsutsugamushi IgG/IgM and R. typhi IgG/IgM are determined to be 20.4, 7.03, 16.8 and 12.5, respectively. The LOD values were all lower than the cut-off values (256, 16, 128, and 64) used for clinical diagnosis, which means that this assay platform can diagnose two typhus diseases with high sensitivity. When the microarray sensors are combined with portable Raman spectrophotometers, which are commercially available worldwide, it is also possible to directly diagnose a patient in the field without sending their blood sample to a hospital.

Year Title Journal Info. etc.
2021  Reproducible and sensitive plasmonic sensing platforms based on Au-nanoparticle-internalized nanodimpled substrate file Advanced Functional Materials, 31, 2105703 (2021)   
2021  SERS-based serodiagnosis of acute febrile diseases using plasmonic nanopopcorn microarray platforms file Biosensors & Bioelectronics, 192, 113525 (2021)   
2021  Coumarin–lipoic acid conjugates on silver nanoparticle-supported nanopipettes for in situ dual-mode monitoring of intracellular Cu(II) and potential chemodynamic therapy applications file Sensors & Actuators B, 344, 130271 (2021)   
2021  SERS-based test strips: Principles, designs and applications file Biosensors & Bioelectronics, 189, 113360 (2021)   
2021  Sensitive detection of SARS-CoV-2 using SERS-based aptasensor file ACS Sensors, 6, 2378-2385 (2021)   
2021  SERS-based microfluidic devices for in vitro diagnostics In "Principles and Clinical Diagnostic Applications of
Surface-Enhanced Raman Spectroscopy" Book Chapter 7, Elsevier, in press. 
Book Chapter 
2021  SERS-based Dual-flow Lateral Flow Assay Sensor for the Ultrasensitive Detection of Thyroid-Stimulating Hormone file Analytical Chemistry, 93, 6673-6681 (2021)   
2021  Molecular Imprinting: Green Perspectives and Strategies file Advanced Materials, 33, 2100543 (2021)   
2021  Recent advances in sensitive surface-enhanced Raman scattering-based lateral flow assay platforms for point-of-care diagnostics of infectious diseases file Sensors & Actuators B, 329, 129214 (2021)  Invited Review 
2021  Determination of total iron-binding capacity of transferrin using metal organic framework-based surface-enhanced Raman scattering spectroscopy file J. Raman Spectrosc., 52, 506-515 (2021)  Invited Paper 
2021  A Wearable Surface-Enhanced Raman Scattering Sensor for Label-Free Molecular Detection file ACS Applied Materials & Interfaces, 13, 3024-3032 (2021)   
2020  Recent advances in surface-enhanced Raman scattering-based microdevices for point-of-care diagnosis of viruses and bacteria file Nanoscale, 12, 21560 (2020)  Invited Review 
2020  SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A file Biosensors & Bioelectronics, 167, 112496 (2020)   
2020  SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing file Talanta, 220, 121397 (2020)   
2020  Improvement of reproducibility and thermal stability of surface-enhanced Raman scattering-based lateral flow assay strips using silica-encapsulated gold nanoparticles file Sensors & Actuators: B. Chemical, 321, 128521 (2020)   
2020  SERS biosensors for ultrasensitive detection of multiple biomarkers expressed in cancer cells file Biosensors & Bioelectronics, 164, 112326 (2020)   
2020  Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities file Biosensors & Bioelectronics, 165, 112282 (2020)   
2020  Multi-emitting fluorescence sensor of MnO2-OPD-QDs for the multiplexed and visual detection of ascorbic acid and alkaline phosphatase file Journal of Materials Chemistry C, 8, 5554-5561 (2020)   
2020  Analysis of deoxyribonuclease activity by conjugation-free fluorescence polarization in sub-nanoliter droplets file Analyst, 145, 3222-3228 (2020)  Cover page 
2020  Present and Future of Surface-Enhanced Raman Scattering file ACS Nano, 14, 28-117 (2020)