Year 2022 
Journal Info. Analytical Chemistry, 94, 6463-6472 (2022) 
Author D. N. Ngo, X. V. T. T. Ho, G. Kim, M. S. Song, M. R. Kim, J. Choo*, S. –W. Joo*, S. Y. Lee* 


Raman thermometry based on surface-enhanced Raman scattering has been developed using nanopipettes in cancer cell photothermal therapy (PTT). Gold nanorods (AuNRs) are robustly epoxied on glass pipettes with a high surface coverage of ∼95% and less than 10 nm-wide nanogaps for intracellular thermometry and photothermal cancer therapy. The temperature changes could be estimated from the N≡C band shifts of 4-fluorophenyl isocyanide (FPNC)-adsorbed AuNRs on the Raman thermometry nanopipette (RTN) surfaces. An intracellular temperature change of ∼2.7 °C produced by altering the [Ca2+] in A431 cells was detected using the RTN in vitro, as checked from fura-2 acetoxymethyl ester (fura-2 AM) fluorescence images. For in vivo experiments, local temperature rises of ∼19.2 °C were observed in the mouse skin, whereas infrared camera images could not tract due to spatial resolution. In addition, a tumor growth suppression was observed in the PTT processes after an administration of the three AuNR-coated nanopipettes combined with a 671 nm laser irradiation for 5 min in 30 days. These results demonstrate not only the localized temperature sensing ability of FPNC-tagged AuNR nanopipettes in cell biology but also anti-cancer effects in photothermal cancer therapy.


Year Title Journal Info. etc.
2022  Rapid and sensitive detection of lysophosphatidylcholine using Zwitterionic polydiacetylene liposome vesicles and a microfluidic gradient sensor Sensors & Actuators B, in press.   
2022  Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza A virus Nano Convergence, in press.   
2022  Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor file Chemical Engineering Journal, 446, 137085 (2022)   
2022  A novel polymer-based nitrocellulose platform for implementing multiplexed microfluidic paper-based enzyme-linked immunosorbent assay file Microsystems & Nanoengineering, 8, 53 (2022)   
2022  Raman thermometry nanopipettes in cancer photothermal therapy file Analytical Chemistry, 94, 6463-6472 (2022)   
2022  Fluorescent probes for biomolecule detection under environmental stress file Journal of Hazardous Materials, 431, 128527 (2022)   
2022  Early and direct detection of bacterial signaling molecules through one-pot Au electrodeposition onto paper-based 3D SERS substrates file Sensors & Actuators B, 358, 131504 (2022)   
2022  iSERS Bioassays In “World Scientific Reference on Plasmonic Nanomaterials :
Principles, Design and Bio-applications. Vol. 5: Plasmonics in Diagnostics
and Therapy” Book Chapter 7, pp 239-265, World Scientific (2022) 
Book Chapter 
2022  Surface-Enhanced Raman Scattering-based Immunoassay for Severe Acute Respiratory Syndrome Coronavirus 2 file Biosensors & Bioelectronics, 202, 114008 (2022)   
2022  SERS-based dual-mode DNA aptasensors for rapid classification of SARS-CoV-2 and influenza A/H1N1 infection file Sensors & Actuators B, 355, 131324 (2022)   
2022  SERS-PCR Assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates file Biosensors & Bioelectronics, 197, 113736 (2022)   
2022  SERS-based microfluidic devices for in vitro diagnostics In "Principles and Clinical Diagnostic Applications of Surface-Enhanced
Raman Spectroscopy" Book Chapter 7, pp 281-302, Elsevier (2022) 
Book Chapter 
2021  Development of SERS-based immunoassay platforms using hollow Au nanostars for reliable SARS-CoV-2 diagnosis file Bull. Kor. Chem. Soc., 42, 1699-1705 (2021)   
2021  Reproducible and sensitive plasmonic sensing platforms based on Au-nanoparticle-internalized nanodimpled substrate file Advanced Functional Materials, 31, 2105703 (2021)  Cover page 
2021  SERS-based serodiagnosis of acute febrile diseases using plasmonic nanopopcorn microarray platforms file Biosensors & Bioelectronics, 192, 113525 (2021)   
2021  Coumarin–lipoic acid conjugates on silver nanoparticle-supported nanopipettes for in situ dual-mode monitoring of intracellular Cu(II) and potential chemodynamic therapy applications file Sensors & Actuators B, 344, 130271 (2021)   
2021  SERS-based test strips: Principles, designs and applications file Biosensors & Bioelectronics, 189, 113360 (2021)   
2021  Sensitive detection of SARS-CoV-2 using SERS-based aptasensor file ACS Sensors, 6, 2378-2385 (2021)   
2021  SERS-based Dual-flow Lateral Flow Assay Sensor for the Ultrasensitive Detection of Thyroid-Stimulating Hormone file Analytical Chemistry, 93, 6673-6681 (2021)   
2021  Molecular Imprinting: Green Perspectives and Strategies file Advanced Materials, 33, 2100543 (2021)