Dual-Function SERS Microdroplet Sensor for Rapid Differentiation of Influenza A and SARS-CoV-2

Abstract

This study presents the development of a dualfunction microdroplet sensor utilizing surface-enhanced Raman scattering (SERS) technology to identify and quantify Influenza A and COVID-19 viruses. The proposed microfluidic device incorporates compartments for two-phase segmented droplet generation, merging, splitting, and detection. Both viral strains were identified by isolating magnetic antibody−antigen complexes from the liquid medium using a magnetized bar embedded in the microfluidic channel. Concurrent Raman spectroscopic readings were obtained as suspended droplets containing residual SERS-active nanoparticles traversed the interrogation zone of the focused laser beam. Precise quantitative analysis was accomplished by correcting characteristic Raman peak intensities for both viruses with internal standards, while ensemble averaging Raman signals from multiple droplets ensured high reproducibility. This dualfunction SERS microdroplet sensor represents a novel in vitro diagnostic approach capable of rapidly distinguishing between COVID-19 and Influenza A with high sensitivity and reproducibility. When coupled with a portable Raman spectrophotometer, the device shows significant potential as a diagnostic tool for swift and in situ detection of both viral pathogens.