Use of surface-enhanced Raman scattering to quantify EGFR markers uninhibited by cetuximab antibodies

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Year 2014 
Journal Info. Biosensors and Bioelectronics, 60, 358–365 (2014). 
Author Eunsu Chung, Jiyoung Lee, Jimin Yu, Sangyeop Lee, Jin Hyun Kang, Il Yup Chung and Jaebum Choo* 
etc.  

Abstract

Epidermal growth factor receptor (EGFR) has been recognized as an important prognostic marker expressed in cancer cells because its activation is associated with key features of cancer including tumor growth, survival, angiogenesis, and metastasis. Cetuximab is the first monoclonal antibody drug that targets EGFR overexpressed in cancer cells. It easily binds to EGFR, thereby down-regulating the receptor, blocking EGFR-mediated tyrosine kinase activity, and inhibiting cellular proliferation. Thus, EGFR–cetuximab binding can be quantified to monitor receptor status and the prognosis of cancer therapy. In this work, we report using SERS imaging to assess the inhibitory effect of cetuximab on EGFR expressed on cancer cells. From SERS mapping images using silica-encapsulated gold nanotags, the localized spatial distribution of EGFR that was not inhibited by cetuximab could be determined. Furthermore, EGFR expression could be accurately quantified through the statistical analysis of surface-enhanced Raman scattering (SERS) spectral data. Our experimental data demonstrate the feasibility of SERS imaging to improve the prognostic efficacy of cetuximab treatment.

 

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Year Title Journal Info. etc.
2014  Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging file Biosensors & Bioelectronics, 51, 238-243 (2014).   
2014  SERS-based competitive immunoassay of troponin I and CK-MB markers for early diagnosis of acute myocardial infarction file Chemical Communications, 50, 1058-1060 (2014).  Cover page 
2014  Quantitative Analysis of Disease Biomarkers Using Surface-Enhanced Raman Scattering Spectroscopy file In “Optical Spectroscopy and Computational Methods in Biology and Medicine”
Book Chapter 14 (2014). Springer 		 Book Chapter 
2014  Synthesis, Characterization and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing file Macromolecular Rapid Communications, 35, 56-65 (2014).   
2014  Detection of the mycotoxin citrinin using silver substrates and Raman spectroscopy file Journal of Hazardous Materials, 265, 89-95 (2014).   
2014  Ultrasensitive trace analysis for 2,4,6-trinitrotoluene using nano-dumbbell surface-enhanced Raman scattering hot spots file Analyst, 139, 807-812 (2014).   
2014  FITC functionalized magnetic core–shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols file Sensors and Actuators B, 193, 857-863 (2014).   
2014  One-Step Detection of Melamine in Milk by Hollow Gold Chip Based on Surface-enhanced Raman Scattering file Talanta, 122, 80-84 (2014).   
2014  Gold Nanorod-Assembled PEGylated Graphene-Oxide Nanocomposites for Photothermal Cancer Therapy file Photochemistry and Photobiology, 90, 659–666 (2014).   
2014  Interactions between the Antifungal Drug Myclobutanil and Gold and Silver Nanoparticles in Penicillium digitatum Investigated by Surface-Enhanced Raman Scattering file Applied Spectroscopy, 136, 3833-3841 (2014).   
2014  Gold Nanoparticle Silica Nanopeapods file Journal of the American Chemical Society, 136, 3833-3841 (2014).   
2014  Adsorption and desorption of tyrosine kinase inhibitor erlotinib on gold nanoparticles file Journal of Colloid and Interface Science, 425, 96–101 (2014).   
2014  A Flow Cytometry–based Submicron-Sized Bacterial Detection System using a Movable Virtual Wall file Lab on a Chip, 14, 2327–2333 (2014).   
2014  Use of surface-enhanced Raman scattering to quantify EGFR markers uninhibited by cetuximab antibodies file Biosensors and Bioelectronics, 60, 358–365 (2014).   
2014  Real-time analysis of diaquat dibromide monohydrate in water with a SERS-based integrated microdroplet sensor file Nanoscale, 6, 8781–8786 (2014).   
2014  SERS-based immunoassay of anti-cyclic citrullinated peptide for early diagnosis of rheumatoid arthritis file RSC Advances, 4, 32924-32927 (2014).   
2014  Colloidal gold nanoparticle conjugates of gefitinib file Colloids and Surfaces B: Biointerfaces, 123, 61–67 (2014)   
2014  Biosensors Using Surface-Enhanced Raman Scattering file In “Encyclopedia of Microfluidics and Nanofluidics”, pp. 1-6 (2014): Springer.  Book Chapter 
2013  Trace analysis of mercury (II) ions using aptamer-modified Au/Ag core-shell nanoparticles and SERS spectroscopy in microdroplet channel file Lab on a Chip, 13, 260-266 (2013).   
2013  SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres file Physical Chemistry Chemical Physics, 15, 5379-5385 (2013).   
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