| Year | 2014 |
|---|---|
| Journal Info. | Sensors and Actuators B, 193, 857-863 (2014). |
| Author | Ling Chen, Jinhua Li, Ghagha Wang, Wenhui Lu, Aiguo Wu, Jaebum Choo, Lingxin Chen* |
| etc. |
Abstract
A sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluores-cein isothiocyanate (FITC) functionalized magnetic core–shell Fe3O4/Ag hybrid nanoparticles. Ag coatedmagnetic Fe3O4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surfaceof core–shell nanoparticles by Ag–SCN linkage and then the fluorescence of FITC was quenched. Uponaddition of molecular biothiols, since the Ag S bond is stronger than Ag–SCN, a place-displacementbetween thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluo-rescence “off-on” probe was attained by virtue of biothiols, so after magnetic separation, the fluorescencesignal change of FITC in clear solution could be employed for quantitative determination of typicalmolecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained withthe detection limits of 10 nM and 20 nM for GSH and Cys, respectively. As well as, the assay strategy pre-sented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocalimaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable forimaging molecular thiols in biological systems.
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