| Year | 2014 |
|---|---|
| Journal Info. | Macromolecular Rapid Communications, 35, 56-65 (2014). |
| Author | Chan Woo Jung, Ghulam Jalani, Juhui Ko, Jaebum Choo and Dong Woo Lim* |
| etc. |
Abstract
Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are fi rst developed for fl uorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide- co -acrylic acid) is chemically modifi ed with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modifi ed polymer solutions containing two different fl uorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specifi c molecular interaction, as confi rmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fl uorescence-based multiplexing biosensing system.
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