| Year | 2015 |
|---|---|
| Journal Info. | Journal of Hazardous Materials, 285, 11-17 (2015) |
| Author | Juhui Ko, Chankil Lee* and Jaebum Choo* |
| etc. |
Abstract
Aflatoxin B1 (AFB1) is a well-known carcinogenic contaminant in foods. It is classified as an extremelyhazardous compound because of its potential toxicity to the human nervous system. AFB1 has also beenextensively used as a biochemical marker to evaluate the degree of food spoilage. In this study, a novelsurface-enhanced Raman scattering (SERS)-based immunoassay platform using silica-encapsulated hol-low gold nanoparticles (SEHGNs) and magnetic beads was developed for highly sensitive detection ofAFB1. SEHGNs were used as highly stable SERS-encoding nano tags, and magnetic beads were used as sup-porting substrates for the high-density loading of immunocomplexes. Quantitative analysis of AFB1 wasperformed by monitoring the intensity change of the characteristic peaks of Raman reporter molecules.The limit of detection (LOD) of AFB1, determined by this SERS-based immunoassay, was determined tobe 0.1 ng/mL. This method has some advantages over other analytical methods with respect to rapidanalysis (less than 30 min), good selectivity, and reproducibility. The proposed method is expected to bea new analytical tool for the trace analysis of various mycotoxins.
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