| Year | 2015 |
|---|---|
| Journal Info. | Bull. Korean Chem. Soc., 36, 822-826 (2015) |
| Author | Juhui Ko, Kiweon Cha, Junho Jeon, Gi-eun Rhie, Jonghoon Choi* and Jaebum Choo* |
| etc. |
Abstract
Anthrax is a highly acute disease caused by the bacterium Bacillus anthracis. In this article, we report a highly sensitive detection technique for pathogenic B. anthracis, based on the use of a surface-enhanced Raman scattering (SERS)-active magnetic sensor. Gold nanoparticles (GNPs) and magnetic beads were used as SERS nanotags and supporting substrates, respectively. Here, an SERS-based competitive immunoassay platform is described for the quantitative evaluation of the anthrax marker. Poly-γ-d-glutamic acid (PGA) capsules were used as a target biomarker for the highly sensitive detection of B. anthracis because PGA is known to be closely associated with the pathogenesis of B. anthracis infection. The SERS-based competitive assay reveals an extraordinarily high sensitivity for PGA with a limit of detection of ~1.0 ng/mL. This work provides a conceptually new immunoassay prototype for fast and sensitive detection of PGA markers.
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