3D droplet microfluidic systems for high-throughput biological experimentation

Manager 2019.03.07 13:23 Views : 1253

Year 2015 
Journal Info. Analytical Chemistry, 87, 10770-10778 (2015) 
Author D. K. Kang, X. Kong, S. Cho, J. Y. Kim, J. B. Edel, S. I. Chang, J. Choo and A. J. deMello* 
etc.  

Abstract

Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D dropletbased microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.

 

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