| Year | 2015 |
|---|---|
| Journal Info. | Journal of Hazardous Materials, 298, 188-194 (2015) |
| Author | J. Kim, Y. Seo, J. Hwang, Y. Jeong, M. P. Hwang, K. H. Lee, J. Choo, G. Rhie, J. H. Jeon, J. Hong and J. Choi* |
| etc. |
Abstract
Francisella tularensis is a human zoonotic pathogen and the causative agent of tularemia, a severe infec-tious disease. Given the extreme infectivity of F. tularensis and its potential to be used as a biologicalwarfare agent, a fast and sensitive detection method is highly desirable. Herein, we construct a noveldetection platform composed of two units: (1) Magnetic beads conjugated with multiple capturing anti-bodies against F. tularensis for its simple and rapid separation and (2) Genetically-engineered apoferritinprotein constructs conjugated with multiple quantum dots and a detection antibody against F. tularensisfor the amplification of signal. We demonstrate a 10-fold increase in the sensitivity relative to traditionallateral flow devices that utilize enzyme-based detection methods. We ultimately envision the use ofour novel nanoprobe detection platform in future applications that require the highly-sensitive on-sitedetection of high-risk pathogens.
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