Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor

Manager 2019.03.07 13:25 Views : 899

Year 2015 
Journal Info. Biosensors & Bioelectronics, 72, 230-236 (2015) 
Author Rongke Gao, Juhui Ko, Kiweon Cha, Jun Ho Jeon, Gi-eun Rhie, Jonghoon Choi, Andrew J. deMello* and Jaebum Choo* 
etc.  

Abstract

We report the application of a fully automated surface-enhanced Raman scattering (SERS)-based solenoid-embedded microfluidic device to the quantitative and sensitive detection of anthrax biomarker poly-γ-D-glutamic acid (PGA) in solution. Analysis is based on the competitive reaction between PGA and PGA-conjugated gold nanoparticles with anti-PGA-immobilized magnetic beads within a microfluidic environment. Magnetic immunocomplexes are trapped by yoke-type solenoids embedded within the device, and their SERS signals were directly measured and analyzed. To improve the acccuracy of measurement process, external standard values for  PGA-free serum were also measured through use of a control channel. This additional measurement greatly improves the reliability of the assay by minimizing the influence of extraneous experimental variables. The limit of detection (LOD) of PGA in serum, determined by our SERS-based microfluidic sensor, is estimated to be 100 pg/mL. We believe that the defined method represents a valuable analytical tool for the detection of anthrax-related aqueous samples.

 

Download PDF = Click Attachment, please.

I want to
List
Year Title Journal Info. etc.
2016  Scalable production of CuInS2/ZnS quantum dots in a two-step droplet-based microfluidic platform file Journal of Materials Chemistry C, 4, 6401-6408 (2016)   
2016  Surface-Enhanced Raman Scattering and Fluorescence-Based Dual Nanoprobes for Multiplexed Detection of Bacterial Pathogens file Journal of Biomedical Nanotechnology, 12, 1938-1951 (2016)   
2016  Early Diagnosis of Influenza Virus A Using SERS-based Lateral Flow Assay file Bulletin of the Korean Chemical Society, 37, 2019-2024 (2016)   
2016  Streptavidin-triggered signal amplified fluorescence polarization for analysis of DNA-protein interaction file Analyst, 141, 6499-6502 (2016)   
2015  Bio-inspired development of a dual-mode nanoparobe for MRI and Raman imaging file Small, 11, 84-89 (2015)   
2015  Large scale plasma patterning of transparent graphene electrode on flexible substrates file Langmuir, 31, 2914-2921 (2015)   
2015  Orientation and Density Control of Bispecific Anti-HER2 Antibody on Functionalized Carbon Nanotubes for Amplifying Effective Binding Reactivity to Cancer Cells file Nanoscale, 7, 6363-6373 (2015)   
2015  Graphene oxide-encoded Ag nanoshells with single-particle detection sensitivity towards cancer cell imaging based on SERRS file Analyst, 140, 3362-3367 (2015)   
2015  Fast and sensitive detection of an anthrax biomarker using SERS-based solenoid microfluidic sensor file Biosensors & Bioelectronics, 72, 230-236 (2015)   
2015  Continuous and low error-rate passive synchronization of pre-formed droplets file RSC Advances, 5, 48399-48405 (2015)   
2015  A Novel Nanoprobe for the Sensitive Detection of Francisella tularensis file Journal of Hazardous Materials, 298, 188-194 (2015)   
2015  Clinical validation of surface-enhanced Raman scattering-based immunoassays in the early diagnosis of rheumatoid arthritis file Analytical & Bioanalytical Chemistry, 407, 8353-8362 (2015)   
2015  Micro 3D cell culture systems for cellular behavior studies: Culture matrices, devices, substrates, and in‐situ sensing methods file Biotechnology Journal, 10, 1682-1685 (2015)   
2015  3D droplet microfluidic systems for high-throughput biological experimentation file Analytical Chemistry, 87, 10770-10778 (2015)   
2015  Surface-Enhanced Raman Scattering-Based Competitive Immunoassay of Poly-γ-D-Glutamic Acid for Highly Sensitive Detection of Anthrax Biomarker file Bull. Korean Chem. Soc., 36, 822-826 (2015)   
2015  Baseline correction using asymmetrically reweighted penalized least squares smoothing file Analyst, 140, 250-257 (2015)   
2015  SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α file Analyst, 140, 2321-2329 (2015)   
2015  Highly sensitive SERS-based immunoassay of aflatoxin B1 using silica-encapsulated hollow gold nanoparticles file Journal of Hazardous Materials, 285, 11-17 (2015)   
2015  Epitaxy-driven vertical growth of single-crystalline cobalt nanowire arrays by chemical vapor deposition file Journal of Materials Chemistry C, 3, 100-106 (2015)   
2015  Surface-enhanced Raman scattering aptasensor for ultrasensitive trace analysis of bisphenol A file Biosensors & Bioelectronics, 64, 560–565 (2015)   
Write Tag