| Year | 2013 |
|---|---|
| Journal Info. | Biosensors & Bioelectornics, 42, 62-67 (2013). |
| Author | Jiyeon Yoon, Namhyun Choi, Juhui Ko, Kihyung Kim, Sangyeop Lee and Jaebum Choo* |
| etc. |
Abstract
This paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (AuNPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA15) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA29)-conjugated AuNPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection(LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27 pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease.
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