A droplet-based microfluidic immunosensor for high efficiency melamine analysis

Manager 2019.03.07 13:38 Views : 1131

Year 2016 
Journal Info. Biosensors & Bioelectronics, 80, 182-186 (2016) 
Author Jae-Won Choi, Kyong-Mi Min, Sundar Hengoju, Gil-Jung Kim, Soo-Ik Chang, Andrew J. deMello, Jaebum Choo* and Hak Yong Kim* 
etc.  

Abstract

We report a droplet-based microfluidic immunosensor for the rapid and accurate detection of melamine, an organic base that has been implicated in widescale adulteration of food products such as milk. Our melamine assay is based on the competitive reaction between native melamine and a melamine-fluorescein isothiocyanate (FITC) conjugate against an anti-hapten antibody. The adoption of fluorescence polarization, allows the quantification of melamine in a more direct and rapid manner than established heterogeneous methods based on liquid chromatography, mass spectrometry, and enzyme-linked immunosorbent assay (ELISA). The detection protocol provides a limit of detection of 300 ppb, which is below the maximum allowable melamine levels (2.5 ppm) defined by the U.S. Food and Drug Administration and the European Commission to a significant extent.

 

 

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Year Title Journal Info. etc.
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2017  Biomedical Applications of SERS Spectroscopy file In “Frontiers and Advances in Molecular Spectroscopy“
Book Chapter 9 (2017), Elsevier. 		 Book Chapter 
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2016  A droplet-based microfluidic immunosensor for high efficiency melamine analysis file Biosensors & Bioelectronics, 80, 182-186 (2016)   
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2016  Highly Sensitive Detection of Hormone Estradiol E2 Using Surface-Enhanced Raman Scattering Based Immunoassays for the Clinical Diagnosis of Precocious Puberty file ACS Applied Materials & Interfaces, 8, 10665-10672 (2016)   
2016  Electrochemical Fabrication of Nanostructures on Porous Silicon for Biochemical Sensing Platforms file Analytical Sciences, 32, 681-686 (2016)   
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