| Year | 2016 |
|---|---|
| Journal Info. | Biosensors & Bioelectronics, 78, 530–537 (2016) |
| Author | Xiuli Fu, Ziyi Cheng, Jimin Yu, Priscilla Choo, Lingxin Chen and Jaebum Choo* |
| etc. |
Abstract
User-friendly lateral flow (LF) strips have been extensively used for point-of-care(POC) self-diagnostics,but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye.To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low con-centration range.Herein, human immunodeficiency virus type1(HIV-1) DNA was chosen as the specific biomarker.Raman reporter-labeled goldnanoparticles(AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker,as opposed to using bare AuNPs in LF strips.It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs.Under optimized conditions,the detection limit of ourSERS-based laeral flow assay was 0.24pg/mL,which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods.These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect abroad range of genetic diseases with high sensitivity.
Download PDF = Click Attachment, please.
