SERS-based genetic assay for amplification-free detection of prostate cancer specific PCA3 mimic DNA

Manager 2019.03.07 13:52 Views : 22499

Year 2017 
Journal Info. Sensors & Actuators B, 251, 302-309 (2017) 
Author Jimin Yu, Jinhyeok Jeon, Namhyun Choi, Jin Oh Lee, Young-Pil Kim and Jaebum Choo* 
etc.  

Abstract

We report the development of amplification-free surface-enhanced Raman scattering (SERS)-based DNA assays for the rapid and highly sensitive detection of prostate cancer antigen 3 (PCA3) mimic DNA. This technique does not require any DNA amplification process using thermo-cycles in conventional polymerase chain reaction (PCR) due to its highly sensitive detection capability. Herein, the PCA3 mimic DNA, composed of 45 nucleotide sequences, was sandwiched between two probe DNA-immobilized particles (ASO735-conjugated detection HGNs and ASO683-immobilized capture magnetic beads) by hybridization reactions. Its quantitative analysis was performed by monitoring the characteristic Raman peak intensity of sandwich DNA complexes. The limit of detection (LOD) is estimated to be 2.7 fM, which is about four orders of magnitude more sensitive than that of conventional PCR. This SERS-based DNA assay technique is expected to be a potentially useful tool for early disease diagnosis.

 

 

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Year Title Journal Info. etc.
2017  Optical nanoprobes for ultrasensitive immunoassay file Analytical Chemistry, 89, 124-137 (2017)   
2017  Simultaneous Detection of Dual Nucleic Acids Using a SERS-based Lateral Flow Biosensor file Analytical Chemistry, 89, 1163-1169 (2017)   
2017  Raman spectrum identification based on the correlation score using weighted segmental hit quality index file Analyst, 142, 380-388 (2017)   
2017  Spectroscopic measurements of interactions between hydrophobic 1-pyrenebutyric acid and silver colloidal nanoparticles file Colloids and Surfaces A, 518, 295-303 (2017)   
2017  Quantum Dot–based Molecularly Imprinted Polymers on Three-Dimensional Origami Paper Microfluidic Chip for Fluorescence Detection of Phycocyanin file ACS Sensors, 2, 243-250 (2017)   
2017  Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy file Chemical Society Reviews, 46, 2237-2271 (2017)   
2017  From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis file Microfluidics and Nanofluidics, 21:58 (2017)   
2017  Simultaneous detection of dual prostate specific antigens using SERS-based immunoassay for accurate diagnosis of prostate cancer file ACS Nano, 11, 4926-4933 (2017)   
2017  SERS-based genetic assay for amplification-free detection of prostate cancer specific PCA3 mimic DNA file Sensors & Actuators B, 251, 302-309 (2017)   
2017  Analysis of ribonuclease activity in sub-nanoliter droplets by label-free fluorescence measurements file Analyst, 142, 2610-2616 (2017)   
2017  Biomedical Applications of SERS Spectroscopy file In “Frontiers and Advances in Molecular Spectroscopy“
Book Chapter 9 (2017), Elsevier. 		 Book Chapter 
2017  Integrated SERS-based microdroplet platform for the automated immunoassay of F1 antigens in Yersinia pestis file Analytical Chemistry, 89, 8413-8420 (2017)   
2017  Surfactant-Free Vapor-Phase Synthesis of Single-Crystalline Gold Nanoplates for Optimally Bioactive Surfaces file Chemistry of Materials, 29, 8747-8756 (2017)   
2016  Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity file Analytical and Bioanalytical Chemistry, 408, 1015-1022 (2016)   
2016  A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA file Biosensors & Bioelectronics, 78, 530–537 (2016)   
2016  A droplet-based microfluidic immunosensor for high efficiency melamine analysis file Biosensors & Bioelectronics, 80, 182-186 (2016)   
2016  Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B file Nanoscale, 8, 11418-11425 (2016)  Cover page 
2016  Wash-free magnetic immunoassay of the PSA cancer marker using SERS and droplet microfluidics file Lab on a Chip, 16, 1022-1029 (2016)   
2016  Highly Sensitive Detection of Hormone Estradiol E2 Using Surface-Enhanced Raman Scattering Based Immunoassays for the Clinical Diagnosis of Precocious Puberty file ACS Applied Materials & Interfaces, 8, 10665-10672 (2016)   
2016  Electrochemical Fabrication of Nanostructures on Porous Silicon for Biochemical Sensing Platforms file Analytical Sciences, 32, 681-686 (2016)   
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