Molecularly imprinted matrix solid-phase dispersion coupled to micellar electrokinetic chromatography for simultaneous determination of triazines in soil, fruit, and vegetable samples

Abstract

A simple and sensitive method for the simultaneous determination of four triazines from soil, strawberry, and tomato samples was developed by selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled tomicellar electrokinetic chromatography (MEKC). Using atrazine as template, the synthesized molecularly imprinted polymers (MIPs) were employed as the dispersion sorbent of MSPD to successfully extract atrazine and its analogs of simazine, ametryn, and propazine from the three different real samples, while matrix interferences were effectively eliminated simultaneously under the optimum extraction conditions. Excellent separation was achieved within 7 min by using an optimized buffer system composed of 30 mmol/L ammonium acetate, 20 mmol/L SDS, and 15% ACN at pH 9.45, obtained by orthogonal design. Good linearity was obtained in a range of 0.5–25 g/g with the correlation coefficients R2 ≥0.9991 except for strawberry sample within 1–25 g/g, and limits of detection were between 12.9–31.5 ng/g in all the three samples. The average recoveries of the four triazines at three different spiked levels were ranged from 53.5 to 98.4% with the relative standard deviations of 1.28–4.89%. This method was proved convenient, costeffective, and environmental benign and could be used as an alternative tool to the existing methods for analyzing the residues of triazines in soil, fruit, and vegetable samples.

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