Date Jan 06, 2021 

중앙대학교 Research News Story에 나노팝콘 기판을 이용한 인플루엔자 A 진단키트가 소개되었습니다.


Researchers develop novel sensor for the improved detection of influenza A viruses, with high reproducibility


Respiratory diseases like influenza can spread rapidly and escalate to global health crises. Thus, to control them, simple yet sensitive detection techniques are needed. Surface-enhanced Raman scattering (SERS) is a potential candidate but currently lacks reproducibility. To overcome this, researchers in Korea have now developed a novel SERS-based sensor that can effectively detect influenza A viral pathogens with high reproducibility. Through this improved method of detection, preventing future outbreaks of respiratory diseases can become easier.



Respiratory viruses are easily transmissible and can spread rapidly, posing a major threat to public health. Thus, improved methods for viral detection are needed to prevent future outbreaks of diseases


Photo courtesy: Arek Socha from Pixabay


Viral respiratory diseases are easily transmissible and can spread rapidly across the globe, causing significant damage. The ongoing covid-19 pandemic is a testament to this. In the past too, other viruses have caused massive respiratory disease outbreaks: for example, a subtype of the influenza virus, the type A H1N1 virus, was responsible for the Spanish flu and the Swine flu outbreaks. Thus, to prevent such health crises in the future, timely and accurate diagnosis of these viruses is crucial. This is exactly what researchers from Korea have attempted to work toward, in their brand-new study. Read on to understand how!


For several decades now, polymerase chain reaction (PCR)-based assays have been the gold standard for detecting influenza viruses. And while these assays are highly sensitive, they can require expensive reagents and complicated protocols. A potentially better alternative can be “surface-enhanced Raman scattering” (SERS). SERS-based assays work by depositing a liquid sample onto a substrate material with a nanostructured noble metal surface. Viral particles from the samples are detected when they hybridize with substrate-bound “aptamers,” molecules that can bind to specific target molecules. This binding is visually detected as a change in “signal intensity,” which decreases as viral load increases due to conformational changes on the substrate. However, a major drawback of these assays is the poor reproducibility of signals from heterogeneous hot junctions (electron-dense regions that contribute to signals).


In an attempt to overcome this challenge, the aforementioned researchers from Chung-Ang University and Korea Institute of Materials Science, led by Professor Jaebum Choo, designed a novel 3D “nano-popcorn” plasmonic substrate. Speaking of the significance of their study published in Biosensors and Bioelectronics, Prof Choo says, “Infectious disease, caused by respiratory influenza, SARS, MERS, and SARS-2 viruses, can spread periodically and are a threat to global health. Our SERS-based aptasensor approach provides a new diagnostic platform for respiratory infectious diseases in the future.”


In their design, the scientists coated two layers of gold particles on a polymer substrate using thermal evaporation sequentially. The two coats were separated by treatment with a compound called “perfluorodecanethiol: (PFDT). The energy difference between PFDT and gold layer was what caused the gold ions to diffuse to the surface, forming nanoparticles that appear as uniformly spaced “popcorns.” This arrangement collectively strengthened the signal intensity that was produced, by generating multiple “hotspots” on the substrate.


The scientists then assessed the performance of the assay using different concentrations of the H1N1 virus. They successfully detected different viral loads in merely 20 minutes and from a minute volume of 3 µL (3 microliters: a 1000th part of 3 ml). Moreover, the system could also classify different strains of influenza viruses accurately and detected H1N1 viruses at a sensitivity three-fold higher than that of the routinely used ELISA tests. Not just this, the assay was found to be highly reproducible. Pleased with the results, Prof. Choo states, “Our assay system enabled the ultrasensitive and reliable analysis of the influenza virus. Such a method would enable early-stage diagnosis, facilitate antiviral treatment initiation, and provide infection surveillance, particularly for those at high risk for virus-related complications.”


In fact, the team is confident that their findings can, someday, be used to fight the current pandemic. Prof. Choo says, “We are currently developing a SERS-based aptasensor for the rapid diagnosis of the coronavirus from human respiratory samples. We are also developing a new diagnostic approach to differentiate between influenza A viruses and coronaviruses.”


Hopefully, the new “nano-popcorn” assay can help to fight many major health crises in the future.









Title of original paper





Hao Chen, Sung-Gyu Park, Namhyun Choi , Joung-Il Moon, Hajun Dang, Anupam Das, Seunghun Lee, Do-Geun Kim, Lingxin Chen, Jaebum Choo



SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A


 Biosensors and Bioelectronics








Department of Chemistry, Chung-Ang University, South Korea

Advanced Nano-Surface Department, Korea Institute of Materials Science (KIMS), South Korea

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Chinese Academy of Sciences, China


About Chung-Ang University


Chung-Ang University is a private comprehensive research university located in Seoul, South Korea. It was started as a kindergarten in 1918 and attained university status in 1953. It is fully accredited by the Ministry of Education of Korea. Chung-Ang University conducts research activities under the slogan of “Justice and Truth.” Its new vision for completing 100 years is “The Global Creative Leader.” Chung-Ang University offers undergraduate, postgraduate, and doctoral programs, which encompass a law school, management program, and medical school; it has 16 undergraduate and graduate schools each. Chung-Ang University’s culture and arts programs are considered the best in Korea.




About Professor Jaebum Choo


Dr Jaebum Choo is a Professor at the Department of Chemistry, Chung-Ang University. He obtained a PhD in Molecular Spectroscopy at Texas A&M University, following which he was a faculty member at Hanyang University. Previously, Prof Choo served as the Director of the Center for Integrated Human Sensing System and Bionano Fusion Technology Program and is currently the Director of the Biomedical Diagnostics Research Center. His research interests include SERS, biosensors, micro-devices, and molecular spectroscopy in addition to developing ultrasensitive optical nano-sensor systems for rapid and sensitive in vitro diagnostics of infectious diseases. He has authored several research articles in peer-reviewed journals.


Title Journal Date
Chung-Ang University Researchers Show A Gold-Paved Path Towards Faster COVID-19 Diagnosis file CAU Research Achievements  Jan 12, 2022 
주재범 교수 연구팀, 코로나19 진단시간 획기적 단축 기술 개발 file CAU News  Dec 08, 2021 
화학과 주재범 교수, 국무총리 표창 수상 file CAU News  Nov 25, 2021 
GFID Magazine '연구성과 우수사례' 소개 file GFID Magazine  Oct 19, 2021 
대한화학회 화학세계 '선도연구센터(ERC)' 소개 file 화학세계  Jul 14, 2021 
중앙대학교 매거진 '선도연구센터(ERC)' 소개 file I'M 여기는 Chung-Ang  Feb 01, 2021 
Fighting Respiratory Virus Outbreaks through “Nano-Popcorn” Sensor-based Rapid Detection file CAU RESEARCH NEWS STORY  Jan 06, 2021 
대한화학회 화학세계 '우수선도연구기관' 소개 file 화학세계  Jul 15, 2020 
과학기술정보통신부 2020년 ‘선도연구센터’ ERC 선정 file CAU News  Jul 01, 2020 
영국왕립화학회 발간 국제학술지 ‘Analyst’ 표지논문 게재 file 대학저널  May 11, 2020 
화학과 주재범 교수, ‘이태규 학술상’ 수상 file CAU News Today  Oct 16, 2019 
곰팡이 독소 1시간내 판별하는 칩 개발 file 뉴스1  Sep 17, 2018 
주재범 교수 영국왕립화학회(RSC) "Analyst 저널 Associate Editor" 선임 file RSC Analyst  Mar 01, 2018 
혈액 몇 방울로 난치성 질환을 진단한다? file HY ERICA 2017년 가을호  Sep 08, 2017 
고위험병원체 빠르게 검출한다 file 아시아경제  Aug 17, 2017 
암 조기 진단·치료 위한 나노물질 개발 file 연합뉴스  Apr 13, 2017 
한양대 주재범 교수, 한국바이오칩학회 학술대상 수상 file NEWSIS  Nov 09, 2016 
Bionano Technology Leading the Medical Industry file NEWS H  Oct 19, 2016 
Top 10 Reviewers for Analyst & Integrative Biology file Analyst  Sep 22, 2016 
Impacts of Hanyang's Research_Hypersensitive Biosensor Platform file INTERNET HANYANG NEWS  Jul 29, 2016 
탄저균 바이오마커 검출용 바이오광센서, 「주간 건강과 질병」에 소개 file 주간 건강과 질병  Jun 09, 2016 
SERS 기반 Lateral flow 식중독균 센서, RSC 학술지 Nanoscale blog 소개 file Nanoscale Blog  Jan 25, 2016 
글로벌연구실 '전국1위' 선정 file 조선에듀  Aug 19, 2015 
한양대-스위스연방공대 글로벌연구실 단계평가 전국1위 선정 file 한양대학교 보도자료  Aug 19, 2015 
백남 석학상 수상 file 한양대학교 보도자료  May 14, 2015 
창의적 인재 양성·융합 연구 통해 세계적인 선도 연구집단으로 성장 file 화학세계  Mar 03, 2014 
심장을 뛰게 하는 시간, 15분 file 한양의 맥박 / 인터넷한양 뉴스 (온 캠퍼스)  Feb 21, 2014 
심근경색 빠르고 정확하게 진단 file 한국경제  Jan 27, 2014 
SBS 모닝와이드, YTN 사이언스 '급성심근경색 진단기술' TV 보도 file SBS 모닝와이드, YTN 사이언스  Jan 22, 2014 
공학과 의학의 만남 '혈액 한 방울로 암 자가진단' file 융·복합 미래 매거진 TECH & BEYOND  Jan 02, 2014 
우수연구센터육성(ERC) 통합형 진단시스템 개발 연구사업 file 한국연구재단 Webzine  Jun 05, 2013 
통합형 휴먼센싱 시스템 ERC, 최우수 센터 선정 file 인터넷 한양 뉴스  Jan 27, 2012 
피 한방울로 질병예측 길 연다 file 디지털타임스  Nov 17, 2011 
“2011년 연구재단 기초연구 우수성과 50선” 선정 file 한국연구재단  Sep 29, 2011 
의학, 공학 연계 - 혁신적 휴먼센싱 기술 사업화 file 한국경제  May 11, 2011 
한국연구재단 융복합연구의 지원현황 및 활성화 방향 file 한국연구재단 e-알리미  Aug 25, 2010 
연구실 소개, 통합형 휴먼센싱 시스템 연구센터 file KBCS 소식지  Mar 20, 2010 
마음을 열어 공학을 보라, 넓고 높고 푸른 생명나노공학과 file HY ERICA 55th  Mar 14, 2010 
의학과 공학의 만남, 융합학문의 새로운 패러다임 제시 file 대한뉴스  Oct 07, 2009 
바이오나노융합분야를 선점할 인재양성의 공간 file Dynamic Korea  Oct 03, 2009 
미래 사회를 여는 신기술, 융합학문의 패러다임을 선도하는 우리대학 file HY ERICA 53th  Sep 07, 2009