Development of SERS-based immunoassay platforms using hollow Au nanostars for reliable SARS-CoV-2 diagnosis

Manager 2021.09.30 10:12 Views : 276

Year 2021 
Journal Info. Bull. Kor. Chem. Soc., 42, 1699-1705 (2021) 
Author Qian Yu, Yixuan Wu, Taejoon Kang* and Jaebum Choo* 
etc.  

Abstract

We developed a novel surface-enhanced Raman scattering (SERS)-based SARS-CoV-2 assay platform using hollow Au nanostars to realize high-sensitivity diagnosis of SARS-CoV-2. The assay was performed using SARS-CoV-2 lysate as the target in a wide dynamic range with virus concentrations ranging from 0 to 104 PFU/ml and has a limit of detection (LOD) of 5.1 PFU/ml. This LOD value shows 100 times and 10 times better sensitivity compared to the LODs measured on the same sample using a commercially available rapid kit and enzyme-linked immunosorbent assay, respectively. Therefore, we believe that this SERS-based SARS-CoV-2 assay platform has high diagnostic accuracy for early or asymptomatic infected patients with low virus concentrations. Furthermore, the probability of a false-negative diagnosis is likely to be very low.

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Year Title Journal Info. etc.
2022  Rapid and sensitive detection of lysophosphatidylcholine using Zwitterionic polydiacetylene liposome vesicles and a microfluidic gradient sensor Sensors & Actuators B, in press.   
2022  Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza A virus Nano Convergence, in press.   
2022  Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor file Chemical Engineering Journal, 446, 137085 (2022)   
2022  A novel polymer-based nitrocellulose platform for implementing multiplexed microfluidic paper-based enzyme-linked immunosorbent assay file Microsystems & Nanoengineering, 8, 53 (2022)   
2022  Raman thermometry nanopipettes in cancer photothermal therapy file Analytical Chemistry, 94, 6463-6472 (2022)   
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2022  Early and direct detection of bacterial signaling molecules through one-pot Au electrodeposition onto paper-based 3D SERS substrates file Sensors & Actuators B, 358, 131504 (2022)   
2022  iSERS Bioassays In “World Scientific Reference on Plasmonic Nanomaterials :
Principles, Design and Bio-applications. Vol. 5: Plasmonics in Diagnostics
and Therapy” Book Chapter 7, pp 239-265, World Scientific (2022) 		 Book Chapter 
2022  Surface-Enhanced Raman Scattering-based Immunoassay for Severe Acute Respiratory Syndrome Coronavirus 2 file Biosensors & Bioelectronics, 202, 114008 (2022)   
2022  SERS-based dual-mode DNA aptasensors for rapid classification of SARS-CoV-2 and influenza A/H1N1 infection file Sensors & Actuators B, 355, 131324 (2022)   
2022  SERS-PCR Assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates file Biosensors & Bioelectronics, 197, 113736 (2022)   
2022  SERS-based microfluidic devices for in vitro diagnostics In "Principles and Clinical Diagnostic Applications of Surface-Enhanced
Raman Spectroscopy" Book Chapter 7, pp 281-302, Elsevier (2022) 		 Book Chapter 
2021  Development of SERS-based immunoassay platforms using hollow Au nanostars for reliable SARS-CoV-2 diagnosis file Bull. Kor. Chem. Soc., 42, 1699-1705 (2021)   
2021  Reproducible and sensitive plasmonic sensing platforms based on Au-nanoparticle-internalized nanodimpled substrate file Advanced Functional Materials, 31, 2105703 (2021)  Cover page 
2021  SERS-based serodiagnosis of acute febrile diseases using plasmonic nanopopcorn microarray platforms file Biosensors & Bioelectronics, 192, 113525 (2021)   
2021  Coumarin–lipoic acid conjugates on silver nanoparticle-supported nanopipettes for in situ dual-mode monitoring of intracellular Cu(II) and potential chemodynamic therapy applications file Sensors & Actuators B, 344, 130271 (2021)   
2021  SERS-based test strips: Principles, designs and applications file Biosensors & Bioelectronics, 189, 113360 (2021)   
2021  Sensitive detection of SARS-CoV-2 using SERS-based aptasensor file ACS Sensors, 6, 2378-2385 (2021)   
2021  SERS-based Dual-flow Lateral Flow Assay Sensor for the Ultrasensitive Detection of Thyroid-Stimulating Hormone file Analytical Chemistry, 93, 6673-6681 (2021)   
2021  Molecular Imprinting: Green Perspectives and Strategies file Advanced Materials, 33, 2100543 (2021)   
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