Year 2022 
Journal Info. Biosensors & Bioelectronics, 197, 113736 (2022) 
Author Yixuan Wu, Hajun Dang, Sung-Gyu Park, Lingxin Chen* and Jaebum Choo* 


The reverse transcription-polymerase chain reaction (RT-PCR) method has been adopted worldwide to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although this method has good sensitivity and specificity, there is a need to develop a more rapid diagnostic technology, given the virus’s rapid spread. However, the RT-PCR method takes a long time to diagnose SARS-CoV-2 because of the required thermocycling steps. Therefore, we developed a surface-enhanced Raman scattering (SERS)-PCR detection method using an AuNP-internalized Au nanodimple substrate (AuNDS) to shorten the diagnosis time by reducing the number of thermocycling steps needed to amplify the DNA. For the representative target markers, namely, the envelope protein (E) and RNA-dependent RNA polymerase (RdRp) genes of SARS-CoV-2, 25 RT-PCR thermocycles are required to reach a detectable threshold value, while 15 cycles are needed for magnetic bead-based SERS-PCR when the initial DNA concentration was 1.00× 105 copies/μL. However, only 8 cycles are needed for the AuNDSbased SERS-PCR. The corresponding detectable target DNA concentrations were 3.36 × 1012, 3.28 × 109, and 2.56 × 107 copies/μL, respectively. Therefore, AuNDS-based SERS-PCR is seen as being a new molecular diagnostic platform that can shorten the time required for the thermocycling steps relative to the conventional RTPCR.


Year Title Journal Info. etc.
2022  Rapid and sensitive detection of lysophosphatidylcholine using Zwitterionic polydiacetylene liposome vesicles and a microfluidic gradient sensor Sensors & Actuators B, in press.   
2022  Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza A virus Nano Convergence, in press.   
2022  Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor file Chemical Engineering Journal, 446, 137085 (2022)   
2022  A novel polymer-based nitrocellulose platform for implementing multiplexed microfluidic paper-based enzyme-linked immunosorbent assay file Microsystems & Nanoengineering, 8, 53 (2022)   
2022  Raman thermometry nanopipettes in cancer photothermal therapy file Analytical Chemistry, 94, 6463-6472 (2022)   
2022  Fluorescent probes for biomolecule detection under environmental stress file Journal of Hazardous Materials, 431, 128527 (2022)   
2022  Early and direct detection of bacterial signaling molecules through one-pot Au electrodeposition onto paper-based 3D SERS substrates file Sensors & Actuators B, 358, 131504 (2022)   
2022  iSERS Bioassays In “World Scientific Reference on Plasmonic Nanomaterials :
Principles, Design and Bio-applications. Vol. 5: Plasmonics in Diagnostics
and Therapy” Book Chapter 7, pp 239-265, World Scientific (2022) 
Book Chapter 
2022  Surface-Enhanced Raman Scattering-based Immunoassay for Severe Acute Respiratory Syndrome Coronavirus 2 file Biosensors & Bioelectronics, 202, 114008 (2022)   
2022  SERS-based dual-mode DNA aptasensors for rapid classification of SARS-CoV-2 and influenza A/H1N1 infection file Sensors & Actuators B, 355, 131324 (2022)   
2022  SERS-PCR Assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates file Biosensors & Bioelectronics, 197, 113736 (2022)   
2022  SERS-based microfluidic devices for in vitro diagnostics In "Principles and Clinical Diagnostic Applications of Surface-Enhanced
Raman Spectroscopy" Book Chapter 7, pp 281-302, Elsevier (2022) 
Book Chapter 
2021  Development of SERS-based immunoassay platforms using hollow Au nanostars for reliable SARS-CoV-2 diagnosis file Bull. Kor. Chem. Soc., 42, 1699-1705 (2021)   
2021  Reproducible and sensitive plasmonic sensing platforms based on Au-nanoparticle-internalized nanodimpled substrate file Advanced Functional Materials, 31, 2105703 (2021)  Cover page 
2021  SERS-based serodiagnosis of acute febrile diseases using plasmonic nanopopcorn microarray platforms file Biosensors & Bioelectronics, 192, 113525 (2021)   
2021  Coumarin–lipoic acid conjugates on silver nanoparticle-supported nanopipettes for in situ dual-mode monitoring of intracellular Cu(II) and potential chemodynamic therapy applications file Sensors & Actuators B, 344, 130271 (2021)   
2021  SERS-based test strips: Principles, designs and applications file Biosensors & Bioelectronics, 189, 113360 (2021)   
2021  Sensitive detection of SARS-CoV-2 using SERS-based aptasensor file ACS Sensors, 6, 2378-2385 (2021)   
2021  SERS-based Dual-flow Lateral Flow Assay Sensor for the Ultrasensitive Detection of Thyroid-Stimulating Hormone file Analytical Chemistry, 93, 6673-6681 (2021)   
2021  Molecular Imprinting: Green Perspectives and Strategies file Advanced Materials, 33, 2100543 (2021)